ova aerosol  (R&D Systems)

Journal: JCI Insight

Article Title: A neutrophil/TGF- β axis limits the pathogenicity of allergen-specific CD4 + T cells

doi: 10.1172/jci.insight.150251

Figure Lengend Snippet: ( A ) Acute inflammatory responses to inhaled LPS/OVA and ASP/OVA. Shown are mean cell numbers ± SEM for the indicated leukocytes in BAL fluid of mice 16 hours after inhalation ( n = 4 mice/group). ( B ) Efficiency of 1A8 Ab–mediated neutrophil depletion in mice inhaling OVA together with the indicated amounts of LPS ( n = 8 mice/group). ( A and B ) Data shown are from single experiments, representative of 2. ( C ) Timeline for neutrophil depletion, followed by LPS/OVA sensitizations and a single OVA challenge. ( D ) Cell numbers for the indicated leukocytes in BAL fluid 2 days after OVA challenge, as shown in C . Values shown represent mean ± SEM, and the data are combined from 2 experiments ( n = 12 mice/group). ( E – G ) Effect of neutrophil depletion on responses to multiple OVA challenges. ( E ) Timeline for neutrophil depletion, allergic sensitization, and multiple OVA challenges. ( F ) Cell numbers for the indicated leukocytes in BAL fluid 2 days after the last of 6 OVA challenges, as shown in E . Values shown represent mean ± SEM, and the data are combined from 3 experiments ( n = 18 mice/group). ( G ) Effect of neutrophil depletion prior to LPS/OVA-mediated sensitization on AHR. Airway resistance values shown represent mean ± SEM and are combined from 2 experiments ( n = 17 mice/group). ( H ) Effect of neutrophil depletion prior to HDE/OVA sensitization. Cell numbers for the indicated leukocytes in BAL fluid of OVA-challenged mice previously sensitized using HDE/OVA as depicted in H. Values shown represent mean ± SEM from 1 of 2 experiments yielding similar results ( n = 6 mice/group). * P < 0.05, ** P < 0.01, *** P < 0.001; 1-way ANOVA with Holm-Šídák multiple-comparison test.

Article Snippet: For allergen challenges, previously sensitized mice were exposed 7 days after the second sensitization to an aerosol of 1% OVA (MilliporeSigma) in PBS for 1 hour on a single occasion or for 30 minutes on multiple consecutive days.

Techniques: Comparison

Journal: JCI Insight

Article Title: A neutrophil/TGF- β axis limits the pathogenicity of allergen-specific CD4 + T cells

doi: 10.1172/jci.insight.150251

Figure Lengend Snippet: ( A ) Timeline for sensitizations to OVA using combinations of LPS (1 and 100 ng) and CXCL1 and harvest. ( B ) Cell numbers for indicated leukocytes in the airway 4 hours after administration of the indicated reagents. Values shown represent mean ± SEM from 1 of 2 experiments yielding similar results ( n = 4 mice/group). * P < 0.05, Kruskal-Wallis 1-way ANOVA with Dunn’s multiple-comparison test. ( C ) Timeline for neutrophil depletion, followed by allergic sensitization and a single OVA challenge. ( D ) Airway inflammation 2 days after OVA challenge of mice treated as shown in C . Values shown represent mean ± SEM from combined data from 2 experiments ( n = 12 mice/group). *** P < 0.001; 1-way ANOVA with Holm-Šídák multiple-comparison test. ( E ) Timeline for multiple OVA challenges following allergic sensitization using combinations of OVA, LPS and CXCL1. ( F ) Airway inflammation 1 day after OVA challenge of mice treated as shown in E . Values shown represent mean ± SEM and are combined from 2 experiments. ( n = 12 mice/group). ** P < 0.01, *** P < 0.001; 1-way ANOVA with Holm-Šídák multiple-comparison test.

Article Snippet: For allergen challenges, previously sensitized mice were exposed 7 days after the second sensitization to an aerosol of 1% OVA (MilliporeSigma) in PBS for 1 hour on a single occasion or for 30 minutes on multiple consecutive days.

Techniques: Comparison

Journal: JCI Insight

Article Title: A neutrophil/TGF- β axis limits the pathogenicity of allergen-specific CD4 + T cells

doi: 10.1172/jci.insight.150251

Figure Lengend Snippet: ( A ) Timeline for neutrophil depletion, inhalation of LPS, transfer of in vitro–generated OVA-specific Th2 cells, and OVA challenges. ( B ) Cell numbers for the indicated leukocytes in BAL fluid 1 day after the final OVA challenge of mice treated as in A ( n = 6 mice/group). ( C ) Effect of neutrophil depletion on cell numbers for the indicated CD4 + Th subsets. Shown are percentages of the indicated CD4 + Th cell subsets in the lungs of mice sensitized with OVA/HDE and challenged with OVA on multiple occasions, as shown in E. ( D ) Effect of neutrophil depletion on Treg functional activity. Relative cell numbers corresponding to each generation of cell division are shown in representative histograms (left). Also shown are compiled data representing proliferative indices (right) as described in Methods. ( E ) Timeline for adoptive transfer of OT-II CD4 + T cells into primary recipients, their OVA sensitization and challenge, harvest of donor CD4 + T cells, and their transfer into secondary recipients. ( F ) Cell numbers for the indicated leukocytes in BAL fluid 1 day following the last challenge of mice treated as shown in E (right). Values shown represent mean ± SEM from 1 of 2 experiments yielding similar results. * P < 0.05, ** P < 0.01, *** P < 0.001; Kruskal-Wallis 1-way ANOVA with Dunn’s multiple-comparison test.

Article Snippet: For allergen challenges, previously sensitized mice were exposed 7 days after the second sensitization to an aerosol of 1% OVA (MilliporeSigma) in PBS for 1 hour on a single occasion or for 30 minutes on multiple consecutive days.

Techniques: In Vitro, Generated, Functional Assay, Activity Assay, Adoptive Transfer Assay, Comparison

Journal: JCI Insight

Article Title: A neutrophil/TGF- β axis limits the pathogenicity of allergen-specific CD4 + T cells

doi: 10.1172/jci.insight.150251

Figure Lengend Snippet: ( A and B ) Effect of TGF-β blockade during sensitization on responses to subsequent OVA challenge. ( A ) Timeline for blockade of TGF-β during allergic sensitization, followed by OVA challenge on a single occasion (top) or multiple occasions (bottom). ( B ) Mean cell numbers ± SEM for eosinophils and neutrophils in BAL fluid of mice that received anti–TGF-β Ab or isotype control (IC) Ab prior to sensitization and were challenged once or on multiple occasions. Data shown are from 1 of 2 experiments yielding similar results ( n = 12 mice/group). ** P < 0.01; Kruskal-Wallis 1-way ANOVA with Dunn’s multiple-comparison test. ( C – E ) Exogenous rmTGF-β reverses the ability of neutrophil blockade to enhance allergic responses. ( C ) Timeline for allergic sensitization to LPS/OVA, neutrophil depletion, inhalation of exogenous rmTGF-β, followed by a single OVA challenge (top) or multiple OVA challenges (bottom). ( D and E ) Effect of rmTGF-β administration on allergic responses of mice undergoing neutrophil depletion. Shown are cell numbers for the indicated cell types in BAL fluid of mice challenged on 6 consecutive days ( D ) or on a single occasion ( E ). Values shown represent mean ± SEM, and data are combined from 2 experiments ( n = 12 mice/group). * P < 0.05, *** P < 0.001; ( A ) Kruskal-Wallis 1-way ANOVA with Dunn’s multiple-comparison test or ( B and C ) 1-way ANOVA with Holm-Šídák multiple-comparison test.

Article Snippet: For allergen challenges, previously sensitized mice were exposed 7 days after the second sensitization to an aerosol of 1% OVA (MilliporeSigma) in PBS for 1 hour on a single occasion or for 30 minutes on multiple consecutive days.

Techniques: Control, Comparison